Sensitive determination of cell number using the CyQUANT cell

Read the absorbance for Cyquant assay using the plate reader with excitation at 485 nm and emission at 530 nm 6 Interpolate the standard curve

This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

cyquant protocol CyQuant® GR Dye : One 25 µL tube 4 Cotton Swabs (Part No described in Assay Protocol References 1 Ridley

cyquant protocol Adherent cells were lysed and quantified by CyQuant® GR Dye as described in the Assay Protocol 1 Hynes, R O Cell 69, 11-25 References 2