Fractionation of Calf Thymus DNA by DEAE Cellulose Column

website deae column chromatography Ion exchange chromatography is usually started at a low ionic strength, at which the proteins bind to the column Then the column is eluted with deae cellulose column Anion-exchange chromatography is a universal purification step, which takes advantage of the highly acidic pI of brachyurins Acid

deae column chromatography Effects of pH on Elution Pattern When the buffer eluent is acidic, four types of proteins are separated using IEC DEAE-825 (a column for weak anion  When serum containing antibodies is applied to DEAE at a slightly acidic pH, the IgG flows through the column while most other serum proteins  Introduction to Ion Exchange Chromatography for Protein Purification

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