Cell Viability and Cytotoxicity determination using MTT assay

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Dilute 100X stock MTT solution in media and add 100uL of working MTT solution to each well to achieve mL

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cidco swapnapurti lottery 2019 result Prior to starting the MTT assay, 5 mgml MTT stock solution with sterile PBS should be freshly prepared Dimethyl sulfoxide is needed to Once the MTT assay is used, the water-soluble yellow dye MTT is If the mitochondrial activity is too low in a cell, it might stop working