TACS MTT Cell Proliferation Assay - Bio-Techne

mtt stock   Dan mtt stock

- add 10 uL of your MTT stock solution and wrap the plate in aluminium foil to prevent exposure to light - after 2-4 hours of incubation (you

MTT Incubation Media Mix 1 ml of MTT Stock solution and 10 ml of Serum-free Culture Medium NOTE: The Williams medium can interefere with formazan color We investigated the applicability and limitations of the MTT assay in representing treatment toxicity, cell viability, and metabolic activity

3-digit midday Do a serial dilution of drug stock Ex: Drug Stock Conc 1uM or 100nM Dilute to: 50nM 25nM etc… Put an additional 3ml PBS in the first well and add MTT stock solution was added into each well The plates were incubated for 4 h at 37◦C Then, 100 μL of the solubilizing solution was added to each well